USVL-370 is a new watermelon breeding line developed by the U.S. Vegetable Laboratory, USDA, with resistance to the Zucchini yellow mosaic virus-Florida strain (ZYMV-FL). The line is homozygous for the recessive eukaryotic elongation factor eIF4E allele, derived from Plant Introduction 595203, and was developed through a breeding process involving phenotypic selection, ELISA testing, and marker-assisted selection.