A study on the genetic diversity of the 3′-terminal region of Papaya ringspot virus (PRSV-W) isolates from watermelon in Oklahoma revealed nucleotide and amino acid sequence identities ranging from 95.2-100% and 97.1-100%, respectively. Phylogenetic analysis showed that the Oklahoma isolates formed distinct clusters based on their locations, contributing valuable insights into the genetic variability of PRSV-W in cucurbit crops.